Research using E. coli laboratory strains that ARE NOT derived from K-12 (e.g., BL21 & Rosetta) requires IBC review. Most research utilizing E. coli laboratory strains that ARE derived from K-12 is exempt from IBC review under the NIH Guidelines. Some research with K-12 may still require IBC review. Non-exempt experiments include, but are not limited to, those involving large volumes of culture (>10 liters) or cloning genes from pathogens or genes involved in synthesis of toxins (see NIH Guidelines section C-II-A). It is the responsibility of the PI to determine whether their experiments with K-12 require review.
E. coli strains derived from K-12; Usually RG1, Exempt (NIH Guidelines III-F-8, Appendix C-II)
| 5K | JC9387 | SURE |
| 58 | JM101 | TB1 |
| 58-161 | JM109 | TG1 |
| AB284 | JM83 | TOP10 |
| AB311 | KP7600 | W1485 |
| AG1 | LE392 | W1485 |
| C600 | M15 | W208 |
| Cavalli Hfr | MB408 | W3110 |
| DH1 | MG1655 | W3110 |
| Dh5-alpha | Novablue | W945 |
| DP50 | P678 | WA704 |
| EMG2 | PA 309 | WA704 |
| EPI100-T1R | REG-12 | WG1 |
| H1443 | S17-1 | XL1-Blue |
| HB101 | SCS-110 | XL10-Gold |
| Hfr 3000 X74 | SM10 | XLOLR |
| Hfr3000 | STBL2 | Y10 |
| HMS 174 | STBL3 | YN2980 |
E. coli strains NOT derived from K-12; Usually RG1, NOT Exempt (NIH Guidelines III-E)
| 25922 | C | Nissile 1917 |
| B | C41 | REG-811 |
| B-3 | C43 | Rosetta |
| B/R | FDA strain Seattle 1946 | TOPP |
| BL21 | K5808 | W |
| BL23 | Mach1 |
Pathogenic E. coli strains; ≥RG2, NOT Exempt (NIH Guidelines III-D)
| E. coli, all strains bearing K1 antigen |
| E. coli enteroaggregative strain (EAEC) |
| E. coli enterohaemorrhagic strain (EHEC) |
| E. coli enteroinvasive strain (EIEC) |
| E. coli enteropathogenic strain (EPEC) |
| E. coli enterotoxigenic strain (ETEC) |
| E. coli Shiga toxin-producing E. coli (O157:H7) |