Center for Gene Regulation in Health and Disease (GRHD)

Facilities manager/Administrator and Facilities

Diane Jackel Greene
Facilities manager/Administrator
Location: SI 201
Phone: (216)875-9824
Fax: (216)687-6972
Manages shared instrumentation within the Center for Gene Regulation in Health and Disease (GRHD). Responsible for general upkeep, routine maintenance, and quality control of high-end instrumentations.
If you are planning to use advanced fluorescence-based Confocal and DeltaVision microscopes, a BD FACSCanto™ benchtop flow cytometer and/or any other shared equipment in the Center, please e-mail and/or call Diane Greene.
Special features of our Nikon A1Rsi Laser Scanning Confocal Microscope include: High resolution galvano scanner (4096x4096 pixels); High speed resonant scanner (30 fps at 512x512 pixels); Transmitted detector for brightfield imaging and Differential Interference Contrast; Spectral imaging capability (can image GFP and Alexa 488 in same sample); DIC optics for transmitted light imaging; Perfect focus system (eliminates focus drift during live imaging); Motorized XYZ stage with piezo control; Photoactivation, photobleaching, and FRET capable; Environmental chamber.
Special features of our Deltavision Deconvolution Microscope include: Filter sets for imaging of DAPI, FITC, Cy3, and Cy5 or similar fluors; Cooled CCD for high speed, low noise image acquisition; DIC optics for transmitted light imaging; Motorized XYZ stage with piezo control; Workstation for image acquisition and deconvolution (upgraded 2018); Environmental chamber.
Nikon DeltaVision
    Nikon A1Rsi confocal microscope     DeltaVision deconvolution microscope
BD FACSCantoTyphoon 9410
     BD FACS Canto II Flow Cytometer          Typhoon 9410 imaging scanner
Typhoon new New GRHD lab
        Amersham Typhoon 5 Imager              Newly renovated GRHD lab 
L90K J2-21
 Beckman Optima L90K ultracentrifuges        Beckman J2-21 centrifuges
OdysseyFrench press
     Odyssey Fc Imaging System                    Thermo Electron French press
Cell culture LS counter
        New cell culture facility                  Beckman LS 6500 scintillation counter
AKTA  Heska
        AKTA Purifier FPLC System         Heska Hematology and Element Analyzers