Chemical and Biomedical Engineering

Mechanistic study of developmental neurotoxicity on 3D cultured stem cell microarrays.

Dr. Moo-Yeal Lee, with his co-investigator Dr. Chandra Kothapalli, was awarded a $1,306,634 grant from NIH/NIEHS. The grant lasts from July 2015 to June 30th, 2019. Their research is titled: Mechanistic study of developmental neurotoxicity on 3D cultured stem cell microarrays. The major goal is to develop neural stem cell microarrays for developmental neurotoxicity assays. 

There is a critical need for improved human toxicology testing to rapidly advance therapeutic drug candidates to preclinical evaluation or to prioritize potential environmental toxicants. The ability to predict developmental neurotoxicity in vivo is of particular importance in identifying drug candidates and environmental toxicants that should be flagged as potentially harmful to the developing brain. The primary objective of our NIH funded research project is to investigate the mechanisms of neurotoxicity on human neural stem cells (NSCs) cultured in three dimension (3D) on a high-throughput microarray chip platform. The outcomes from this work could help in precisely measuring various cellular and molecular pathways involved in human developmental toxicology.