Biological, Geological, and Environmental Sciences

Dr. Sailen Barik

Ph.D., University of Calcutta;
Office Phone: (216) 523-7326
Lab Phone: (216) 523-7562
COSHP Faculty Profile

Research Interests

My laboratory's overall interest is in cardiopulmonary pathology, particularly in relation to inflammation and infection of the Heart and the Lungs. In mechanistic terms, we are interested in understanding the interactions of these organs with specific infectious agents, the molecular signaling pathways that regulate such interactions, and the involvement of RNA interference (RNAi) and microRNA (miRNA) in these processes. The main focus areas are the following: (i) Infection of heart and lung by unicellular protozoan parasites (e.g., Toxoplasma gondii) and RNA viruses; (ii) Suppression of pulmonary innate immunity by RNA viruses, mainly respiratory syncytial virus (RSV); (iii) Reciprocal regulation of cellular miRNA by pathogens, mainly T. gondii and RSV.

Selected Publications

Adams B, Musiyenko A, Kumar R, Barik S. A novel class of dual-family immunophilins. J Biol Chem. 2005; [Epub ahead of print]

Kumar R, Adams B, Musiyenko A, Shulyayeva O, Barik S. The FK506-binding protein of the malaria parasite, Plasmodium falciparum, is a FK506-sensitive chaperone with FK506-independent calcineurin-inhibitory activity. Mol Biochem Parasitol. 2005;141(2):163-73.

Kumar R, Musiyenko A, Barik S. Plasmodium falciparum calcineurin and its association with heat shock protein 90: mechanisms for the antimalarial activity of cyclosporin A and synergism with geldanamycin. Mol Biochem Parasitol. 2005;141(1):29-37.

Bitko V, Musiyenko A, Shulyayeva O, Barik S. Inhibition of respiratory viruses by nasally administered siRNA. Nat Med. 2005;11(1):50-5.

Barik S. Development of gene-specific double-stranded RNA drugs. Ann Med. 2004;36(7):540-51.

Barik S. When proteome meets genome: the alpha helix and the beta strand of proteins are eschewed by mRNA splice junctions and may define the minimal indivisible modules of protein architecture. J Biosci. 2004;29(3):261-73.

Bitko V, Garmon NE, Cao T, Estrada B, Oakes JE, Lausch RN, Barik S. Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection. BMC Microbiol. 2004;4(1):28.

Kumar R, Musiyenko A, Oldenburg A, Adams B, Barik S. Post-translational generation of constitutively active cores from larger phosphatases in the malaria parasite, Plasmodium falciparum: implications for proteomics. BMC Mol Biol. 2004;5(1):6.

Maggon K, Barik S. New drugs and treatment for respiratory syncytial virus. Rev Med Virol. 2004;14(3):149-68.

Barik S. Control of nonsegmented negative-strand RNA virus replication by siRNA. Virus Res. 2004;102(1):27-35.

Kumar R, Musiyenko A, Cioffi E, Oldenburg A, Adams B, Bitko V, Krishna SS, Barik S. A zinc-binding dual-specificity YVH1 phosphatase in the malaria parasite, Plasmodium falciparum, and its interaction with the nuclear protein, pescadillo. Mol Biochem Parasitol. 2004;133(2):297-310.

Kumar R, Musiyenko A, Barik S. The heat shock protein 90 of Plasmodium falciparum and antimalarial activity of its inhibitor, geldanamycin. Malar J. 2003;2(1):30.

Bitko V, Oldenburg A, Garmon NE, Barik S. Profilin is required for viral morphogenesis, syncytium formation, and cell-specific stress fiber induction by respiratory syncytial virus. BMC Microbiol. 2003;3(1):9.

Kumar R, Adams B, Oldenburg A, Musiyenko A, Barik S. Characterisation and expression of a PP1 serine/threonine protein phosphatase (PfPP1) from the malaria parasite, Plasmodium falciparum: demonstration of its essential role using RNA interference. Malar J. 2002;1(1):5.

Bitko V, Barik SPhenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses. BMC Microbiol. 2001;1(1):34.

Dobson S, Kar B, Kumar R, Adams B, Barik SA novel tetratricopeptide repeat (TPR) containing PP5 serine/threonine protein phosphatase in the malaria parasite, Plasmodium falciparum. BMC Microbiol. 2001;1(1):31.

Bitko V, Barik SAn endoplasmic reticulum-specific stress-activated caspase (caspase-12) is implicated in the apoptosis of A549 epithelial cells by respiratory syncytial virus. J Cell Biochem. 2001;80(3):441-54.

Burke E, Mahoney NM, Almo SC, Barik SProfilin is required for optimal actin-dependent transcription of respiratory syncytial virus genome RNA. J Virol. 2000 Jan;74(2):669-75.