Cleveland State University

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Available Technologies - Abstract


SDIFA mass spectrometry

Short Description New technology termed as SDIFA is developed for the improvement of mass resolution of linear matrix-assisted-laser-desorption-ionization time-of-flight mass spectrometry in oligonucleotide analysis.
Abstract The mass resolution and accuracy of delayed extraction matrix assisted desorption/ionization time of flight mass spectrometry is improved by spatially separating the electric field used for driving extraction/acceleration from the sample being analyzed.
Inventor(s) Dr. Baochuan Guo, Ph.D. Professor, Department of Chemistry.
Commercial Applications
  • Due to its superior performance, SDIFA will likely be the method of choice for genotyping of single nucleotide polymorphism (SNPs), the most common genetic variation in the human genome.
  • Excellent resolution in the mass range of 5200-7800 Daltons allows unambiguous identification of any heterozygote samples produced using the pinpoint assays.    
  • Superior resolution for oligonucleotides up to the 62mer makes it possible to directly genotype SNPs from PCR products.
  • Unlike the currently used delayed extraction method, SDIFA allows separation of desorption-ionization from ion extraction and selectively sampling of part of the desorbed ions thereby reducing the initial velocity distribution of the sampled ions and improving resolution. 
IP Information 6,552,335
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Status Available
engaged learning
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Parker Hannifin Hall, 214
2258 Euclid Avenue
Cleveland, OH 44115
Phone: 216.687.5108
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